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The multiple field studies have the advantage of large sample sizes, but there are challenges in the field. Noise levels can vary depending on environmental factors, such as wind speed and direction and time of day. The ambient noise levels used in many studies are often not actual steady-state environmental noise levels (Dunlop et al. 2016), but rather averages or averages over short periods (e.g., Dunlop et al. 2016), which are more likely to be representative of behavioral changes. Sound levels are also often estimated by extrapolation of data from other sources, rather than measured directly at the source. For instance, Dunlop et al. (2016, 2020) obtained ambient noise levels from a local government official and used them to calculate exposure level in humpback whales. Without direct measurements at the source, this can be a significant challenge. The field studies also often lack the ability to control the context of exposure, such as the presence or absence of other disturbance, presence of prey, or certain behaviors that could contribute to stress responses. One of the most recent field studies that was able to control for these factors was Dunlop et al. (2020), who exposed migrating humpback whales to ambient playback of humpback song and compared the responses of whales to those that were exposed to the same song pattern at ambient noise levels. This study showed that exposure to ambient noise did not increase the probability of humpback whales changing their behavior during migration, although the effect of the song playback was not statistically significant.
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