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Most of the people who hike with me carry more than one liter of water and on most trips, it is not cold enough for their water to freeze solid. But in the colder conditions (15º F or lower), the more you carry the harder it is to keep it from freezing.
Important rule: Never allow water to freeze in your water bottles. If you fill a Nalgene with water and it starts to fully freeze up before you can use it, just pour it out to maintain the use of the bottle. If water freezes a full plastic Nalgene it is unlikely you will be able to unfreeze it. The bottle just becomes a useless weight.
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Keep extra unfrozen ice-packs that do not fit in the freezer in the bottom part of the main refrigerator compartment (look back at Figure 6.3). This helps the water in these chilled ice-packs to freeze relatively quickly when you put them into the freezer, and it also helps to keep this section of the refrigerator cold in case of a power failure.
Conditioned ice-packs have first been fully frozen, and then removed from the freezer and left at room temperature for a short time (it may take over 30 minutes if the room is cold). Allow the frozen ice-packs to sit at room temperature until the ice begins to melt and water starts to form. Check to see if each ice-pack has been conditioned properly by shaking it and listening for the sound of water moving inside. This prevents the ice-packs from freezing the vaccines inside a cold box or vaccine carrier, and damaging the freeze-sensitive vaccines.
Studies conducted in many countries on cold chain temperatures have revealed that many vaccines are damaged more severely by freezing than by heat. So it is crucial to use properly conditioned ice-packs or chilled water packs when transporting freeze-sensitive vaccines like pentavalent, PCV10 and TT. Chilled water packs can be made by almost filling the ice-pack containers or ordinary plastic water bottles with water and placing them in the main compartment of the refrigerator for about 24 hours. Using chilled water packs may be more efficient than using conditioned ice-packs, because it takes more electricity, gas or kerosene, and more time, to freeze ice-packs and then condition them. Also, there is a risk that if frozen ice-packs are not conditioned properly, they may expose freeze-sensitive vaccines to damage from freezing during transport in vaccine carriers or cold boxes.
It is important to know about the common cold chain monitoring equipment for keeping a record of the temperature that vaccines and diluents are exposed to during transportation and storage. The items of equipment used are vaccine vial monitors, freeze indicators and thermometers.
A vaccine vial monitor (VVM) is a label that changes colour when the vaccine vial or ampoule has been exposed to temperatures above 8ºC over a period of time. Before opening a vaccine container, the status of the VVM must be checked to see whether the vaccine has been damaged by heat. Manufacturers attach VVMs to vials and ampoules of most vaccines. The VVM is printed on the label or cap, or the neck of ampoules of freeze-dried vaccines (Figure 6.7). It looks like a square inside a circle. As the vaccine vial is exposed to more heat, the square becomes darker.
Freeze indicators are devices used to monitor the exposure of vaccines to freezing. Freeze indicators are packed with batches of freeze-sensitive EPI vaccines (pentavalent, PCV10 and TT), as well as with other freeze-sensitive vaccines such as HepB, which may be used to protect healthcare workers. The most commonly used type of freeze indicator is the freeze-tag (Figure 6.9). This is an irreversible temperature indicator that shows if a product, such as a vaccine, has been exposed to freezing. It consists of an electronic temperature measuring circuit with a liquid crystal display (LCD). A small blinking dot of light in the corner of the display shows that the freeze-tag is functioning correctly.
The shake test is how you check whether freeze-sensitive vaccines (pentavalent, PCV10, TT or HepB) have been subjected to freezing temperatures, which are likely to have damaged them. To perform the shake test, follow the steps below:
All vaccines are heat-sensitive and most are freeze-sensitive. Therefore it is important to monitor the temperature of the refrigerator, so that the vaccines are prevented from becoming too warm or freezing.
A refrigerator works well only if it is properly installed, cleaned and defrosted (ice is removed) regularly. Thick ice in the freezer compartment does not keep a refrigerator cool. Instead, it makes the refrigerator work harder and uses more electricity, gas or kerosene. You should defrost the refrigerator when ice becomes more than 0.5 cm thick, or once a month, whichever comes first. 153554b96e
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